Composite

Part:BBa_K5286004:Design

Designed by: Marten Meckelburg   Group: iGEM24_TUM-Straubing   (2024-10-01)


CAHS94063+His Tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 696
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 696
    Illegal NotI site found at 702
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 696
    Illegal BglII site found at 459
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 696
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 696
    Illegal AgeI site found at 755
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The basic BBa_K5286001 coding sequence part (CAHS94063 from Hypsibius dujardini) was assembled to the pET-16b vector without scars via Gibson Assembly.


Source

This part was assembled by the 2024 iGEM team from TUM campus Straubing. The coding sequence part (CAHS94063 from Hypsibius dujardini) was assembled into the pET-16b vector, which includes the HisTag.

References